# Latest **URL:** https://forum.cadet-web.de/latest.md?page=1 [Latest](https://forum.cadet-web.de/latest.md) · [Categories](https://forum.cadet-web.de/categories.md) · [Tags](https://forum.cadet-web.de/tags.md) **Page:** 2 --- ## [Reproducing Simulated Chromatograms from "Predictive mechanistic modeling of loading and elution in protein A chromatography" article from Lenhoff (2024)](https://forum.cadet-web.de/t/reproducing-simulated-chromatograms-from-predictive-mechanistic-modeling-of-loading-and-elution-in-protein-a-chromatography-article-from-lenhoff-2024/1389)
**Author:** [@2253shubham](https://forum.cadet-web.de/u/2253shubham)\ **Replies:** 2\ **Last updated:** [February 14, 2026, 8:25am UTC](https://forum.cadet-web.de/t/reproducing-simulated-chromatograms-from-predictive-mechanistic-modeling-of-loading-and-elution-in-protein-a-chromatography-article-from-lenhoff-2024/1389 "2026-02-14T08:25:18Z")
Hello community, I am new to CADET modeling and still gaining familiarity with chromatography process modeling. I am currently working on reproducing the simulated chromatograms presented in: “Bhoyar, S.; Kumar, V.; Fos… --- ## [Protein A affinity PCC modelling](https://forum.cadet-web.de/t/protein-a-affinity-pcc-modelling/1345)
**Author:** [@naveen567](https://forum.cadet-web.de/u/naveen567)\ **Replies:** 6\ **Last updated:** [February 6, 2026, 9:21am UTC](https://forum.cadet-web.de/t/protein-a-affinity-pcc-modelling/1345 "2026-02-06T09:21:12Z")
Hi, I am working on modeling Protein A affinity PCC chromatography. Since I am not very familiar with SMB Builder, I tried using the scheduling method. Expected result: I was expecting a proper elution for different c… --- ## [Autoscale population and number of generations](https://forum.cadet-web.de/t/autoscale-population-and-number-of-generations/1377)
**Author:** [@j.schmoelder](https://forum.cadet-web.de/u/j.schmoelder)\ **Replies:** 1\ **Last updated:** [February 5, 2026, 1:52pm UTC](https://forum.cadet-web.de/t/autoscale-population-and-number-of-generations/1377 "2026-02-05T13:52:54Z")
I often find it challenging to determine the optimal population size and number of generations for genetic algorithms. Previously, I relied on a fixed number of generations (typically 64) and scaled the population size l… --- ## [Installing CADET](https://forum.cadet-web.de/t/installing-cadet/1376)
**Author:** [@ollie\_p](https://forum.cadet-web.de/u/ollie_p)\ **Replies:** 1\ **Last updated:** [February 5, 2026, 1:35pm UTC](https://forum.cadet-web.de/t/installing-cadet/1376 "2026-02-05T13:35:19Z")
Expected behavior. CADET installs and works. Actual behavior CADET returns an abundance of errors when running in VSCode jupyter notebooks or spyder python script. How to produce bug (including a minimal reproducible e… --- ## [Excluding components from the Fractionator (divide\_by\_zero error)](https://forum.cadet-web.de/t/excluding-components-from-the-fractionator-divide-by-zero-error/1330)
**Author:** [@101abhinav](https://forum.cadet-web.de/u/101abhinav)\ **Replies:** 3\ **Last updated:** [January 27, 2026, 12:06pm UTC](https://forum.cadet-web.de/t/excluding-components-from-the-fractionator-divide-by-zero-error/1330 "2026-01-27T12:06:03Z")
Expected behavior. Hi, I’m currently checking out the capabilities of the Fractionator object within CADET-Process. I’m working with a model where the first component is Salt, but it does not really make much sense to … --- ## [Modelling a multi-layer depth filter operated at constant superficial velocity in CADET](https://forum.cadet-web.de/t/modelling-a-multi-layer-depth-filter-operated-at-constant-superficial-velocity-in-cadet/1355)
**Author:** [@kautharmaalim](https://forum.cadet-web.de/u/kautharmaalim)\ **Replies:** 1\ **Last updated:** [January 4, 2026, 12:21pm UTC](https://forum.cadet-web.de/t/modelling-a-multi-layer-depth-filter-operated-at-constant-superficial-velocity-in-cadet/1355 "2026-01-04T12:21:49Z")
Hello CADET community, I am working on mechanistic modelling of a depth filtration unit and would appreciate guidance on how best to model it in CADET. The filter is operated at constant velocity (i.e., pressure increa… --- ## [Simultaneous Optimization for multiple components for protein affinity chromatography](https://forum.cadet-web.de/t/simultaneous-optimization-for-multiple-components-for-protein-affinity-chromatography/1350)
**Author:** [@101abhinav](https://forum.cadet-web.de/u/101abhinav)\ **Replies:** 4\ **Last updated:** [December 22, 2025, 8:31am UTC](https://forum.cadet-web.de/t/simultaneous-optimization-for-multiple-components-for-protein-affinity-chromatography/1350 "2025-12-22T08:31:39Z")
Hey there, I was currently trying to simulate the protein CEX chromatography elution curve, for which I reviewed the case studies provided in the documentation. I successfully simulated my desired curve and optimised pa… --- ## [PhD position: Modeling carbon allocation in plant root networks (FZ Jülich)](https://forum.cadet-web.de/t/phd-position-modeling-carbon-allocation-in-plant-root-networks-fz-julich/1344)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [December 5, 2025, 12:34pm UTC](https://forum.cadet-web.de/t/phd-position-modeling-carbon-allocation-in-plant-root-networks-fz-julich/1344 "2025-12-05T12:34:23Z")
FZ Jülich is recruiting a PhD researcher for VICAR, a project on in vivo quantification and simulation of carbon allocation in plant root architectures. The position involves developing mechanistic transport models using… --- ## [Postdoc position: Chromatographic separation of biobased molecules (Finland)](https://forum.cadet-web.de/t/postdoc-position-chromatographic-separation-of-biobased-molecules-finland/1343)
**Author:** [@j.schmoelder](https://forum.cadet-web.de/u/j.schmoelder)\ **Replies:** 0\ **Last updated:** [December 5, 2025, 8:46am UTC](https://forum.cadet-web.de/t/postdoc-position-chromatographic-separation-of-biobased-molecules-finland/1343 "2025-12-05T08:46:55Z")
Our friend Tuomo Sainio asked us to forward this open position to anyone who’s interested in spending some time in lovely Finland, working on the design and analysis of separation processes using data-driven or AI-assist… --- ## [Modeling IEC using SMA and GRM](https://forum.cadet-web.de/t/modeling-iec-using-sma-and-grm/1342)
**Author:** [@yqabil](https://forum.cadet-web.de/u/yqabil)\ **Replies:** 1\ **Last updated:** [December 4, 2025, 3:28pm UTC](https://forum.cadet-web.de/t/modeling-iec-using-sma-and-grm/1342 "2025-12-04T15:28:53Z")
Hello everyone, New to CADET here, I work as a downstream processing specialist particularly Monoclonals purification. I am trying to first understand CADET’s architecture and i have successfully simulated adsorption us… --- ## [Phase of Modifiers in CADET](https://forum.cadet-web.de/t/phase-of-modifiers-in-cadet/1336)
**Author:** [@nyousuf](https://forum.cadet-web.de/u/nyousuf)\ **Replies:** 7\ **Last updated:** [November 26, 2025, 6:19am UTC](https://forum.cadet-web.de/t/phase-of-modifiers-in-cadet/1336 "2025-11-26T06:19:02Z")
Hi All, I have a background in CFD, but I am new to the CADET-Core modelling. I wanted to ask few questions regarding the binding kinetics implemented in CADET For the SMA model (Steric Mass Action — CADET), how does … --- ## [Extracting Data from UNICORN Files](https://forum.cadet-web.de/t/extracting-data-from-unicorn-files/635)
**Author:** [@s.leweke](https://forum.cadet-web.de/u/s.leweke)\ **Replies:** 28\ **Last updated:** [November 23, 2025, 5:17am UTC](https://forum.cadet-web.de/t/extracting-data-from-unicorn-files/635 "2025-11-23T05:17:05Z")
Hi Forum, I’m looking for some code (language doesn’t matter so much) to read in UNICORN files and export online signals (UV, conductivity, etc.). Exporting control data (e.g., flow rates) would be a nice as well. Do y… --- ## [Introducing Office Hours](https://forum.cadet-web.de/t/introducing-office-hours/518)
**Author:** [@j.schmoelder](https://forum.cadet-web.de/u/j.schmoelder)\ **Replies:** 0\ **Last updated:** [November 12, 2025, 11:00pm UTC](https://forum.cadet-web.de/t/introducing-office-hours/518 "2025-11-12T23:00:00Z")
Having trouble with CADET? Want to get started implementing custom models? Interested in supporting the project? We’re introducing monthly community office hours where you can ask all of your questions. Join us every f… --- ## [AttributeError: 'NoneType' object has no attribute '\_get\_values\_from\_container' (parameter fitting attempt)](https://forum.cadet-web.de/t/attributeerror-nonetype-object-has-no-attribute-get-values-from-container-parameter-fitting-attempt/1319)
**Author:** [@AN\_IA](https://forum.cadet-web.de/u/AN_IA)\ **Replies:** 5\ **Last updated:** [November 11, 2025, 4:02pm UTC](https://forum.cadet-web.de/t/attributeerror-nonetype-object-has-no-attribute-get-values-from-container-parameter-fitting-attempt/1319 "2025-11-11T16:02:13Z")
Expected behavior. Hello everyone, I have tried to implement parameter fitting into my model. I get this error message: (AttributeError: 'NoneType' object has no attribute '\_get\_values\_from\_container'). Expected beha… --- ## [Solver.nthreads not working in v5.0](https://forum.cadet-web.de/t/solver-nthreads-not-working-in-v5-0/1087)
**Author:** [@a.moser](https://forum.cadet-web.de/u/a.moser)\ **Replies:** 13\ **Last updated:** [October 31, 2025, 6:27am UTC](https://forum.cadet-web.de/t/solver-nthreads-not-working-in-v5-0/1087 "2025-10-31T06:27:32Z")
Hi All, We’ve noticed that it seems like the solver.nthreads variable isn’t actually changing the number of threads in v5.0. We saw this in CPU monitoring while running simulations in v4.4 vs v5.0. Is there a change th… --- ## [Experimental reference data for modeling project](https://forum.cadet-web.de/t/experimental-reference-data-for-modeling-project/1332)
**Author:** [@robertb](https://forum.cadet-web.de/u/robertb)\ **Replies:** 1\ **Last updated:** [October 23, 2025, 3:54pm UTC](https://forum.cadet-web.de/t/experimental-reference-data-for-modeling-project/1332 "2025-10-23T15:54:33Z")
Hi, First of all, sorry if this is an inappropriate question, but I wasnt sure where else to reach out. I am doing a project at university where I will be using and showcasing CADET for digital process development with… --- ## [How to plot only results of last optimization step/Get number of current optimization step?](https://forum.cadet-web.de/t/how-to-plot-only-results-of-last-optimization-step-get-number-of-current-optimization-step/1328)
**Author:** [@AN\_IA](https://forum.cadet-web.de/u/AN_IA)\ **Replies:** 1\ **Last updated:** [October 13, 2025, 7:51am UTC](https://forum.cadet-web.de/t/how-to-plot-only-results-of-last-optimization-step-get-number-of-current-optimization-step/1328 "2025-10-13T07:51:44Z")
Hello, for my parameter estimation, I would like to save the solution and plot of only the last and final optimization generation. (I am currently getting a lot of pareto results, and saving them all for all optimizatio… --- ## [How to solve equilibrium reactions numerically stable and efficient with Sundials?](https://forum.cadet-web.de/t/how-to-solve-equilibrium-reactions-numerically-stable-and-efficient-with-sundials/1314)
**Author:** [@Frank\_Zillmann](https://forum.cadet-web.de/u/Frank_Zillmann)\ **Replies:** 4\ **Last updated:** [October 10, 2025, 10:21am UTC](https://forum.cadet-web.de/t/how-to-solve-equilibrium-reactions-numerically-stable-and-efficient-with-sundials/1314 "2025-10-10T10:21:50Z")
Dear CADET-Team, I am currently working on my bachelor’s thesis at the chair of Bioseparation Engineering at TU Munich on a (separately written) simulation of “High Gradient Magnetic Separation”. As you do in CADET-Core… --- ## [Fit sum of species to two experiments simultaneously (using CADET-Match)](https://forum.cadet-web.de/t/fit-sum-of-species-to-two-experiments-simultaneously-using-cadet-match/1324)
**Author:** [@AN\_IA](https://forum.cadet-web.de/u/AN_IA)\ **Replies:** 4\ **Last updated:** [October 4, 2025, 8:03am UTC](https://forum.cadet-web.de/t/fit-sum-of-species-to-two-experiments-simultaneously-using-cadet-match/1324 "2025-10-04T08:03:42Z")
Hello everyone, I have a component system consisting of 6 components. Three of them are accompanying components, the other three are species of my component of interest, ‘IA’. I have experimental data for the sum of the… --- ## [Fractionator print all components](https://forum.cadet-web.de/t/fractionator-print-all-components/1320)
**Author:** [@sasha\_onfleek1](https://forum.cadet-web.de/u/sasha_onfleek1)\ **Replies:** 4\ **Last updated:** [October 2, 2025, 4:01pm UTC](https://forum.cadet-web.de/t/fractionator-print-all-components/1320 "2025-10-02T16:01:23Z")
Hey everyone, Does anyone know how, when using the fractionator, I can print the information for all the different components, rather than for just one? This is what I’m using now: frac = Fractionator(results, componen… --- ## [Affinity chromatography in CADET-Process](https://forum.cadet-web.de/t/affinity-chromatography-in-cadet-process/1000)
**Author:** [@Ronan1234](https://forum.cadet-web.de/u/Ronan1234)\ **Replies:** 18\ **Last updated:** [October 1, 2025, 6:24am UTC](https://forum.cadet-web.de/t/affinity-chromatography-in-cadet-process/1000 "2025-10-01T06:24:29Z")
Reading this thread: Protein A affinity help and simulation error It seems that the way to elution in affinity chromatography is to have the parameters of the binding model altered during elution. How can I model this i… --- ## [CADET Workshop 2025 Announcement](https://forum.cadet-web.de/t/cadet-workshop-2025-announcement/1156)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 1\ **Last updated:** [September 25, 2025, 12:35pm UTC](https://forum.cadet-web.de/t/cadet-workshop-2025-announcement/1156 "2025-09-25T12:35:11Z")
Announcement We are happy to announce the seventh CADET tutorial and user workshop on November 5-7, 2025. On the first and second day, we will offer introductory and advanced tutorials for new CADET users. The third day … --- ## [CADET installation issue mac M3](https://forum.cadet-web.de/t/cadet-installation-issue-mac-m3/1157)
**Author:** [@naveen567](https://forum.cadet-web.de/u/naveen567)\ **Replies:** 4\ **Last updated:** [September 19, 2025, 7:56am UTC](https://forum.cadet-web.de/t/cadet-installation-issue-mac-m3/1157 "2025-09-19T07:56:38Z")
Hi all, I attempted to install CADET-core through conda-forge but was unable to do so. When I tried using the Immudzen channel, I encountered an error when importing a cadet from cadet-process. I would appreciate your a… --- ## [Printing Solution for particle (how much adsorbed on the particle)](https://forum.cadet-web.de/t/printing-solution-for-particle-how-much-adsorbed-on-the-particle/1310)
**Author:** [@kautharmaalim](https://forum.cadet-web.de/u/kautharmaalim)\ **Replies:** 2\ **Last updated:** [September 10, 2025, 11:19am UTC](https://forum.cadet-web.de/t/printing-solution-for-particle-how-much-adsorbed-on-the-particle/1310 "2025-09-10T11:19:02Z")
Hi Cadet users, I want to print an array showing how much is bound across the column length. I used these lines of code to return data that I want to print # Return data model.root.input\['return'\].split\_components\_data… --- ## [Fitting reaction parameters to a set of experimental data](https://forum.cadet-web.de/t/fitting-reaction-parameters-to-a-set-of-experimental-data/1296)
**Author:** [@Adrian](https://forum.cadet-web.de/u/Adrian)\ **Replies:** 9\ **Last updated:** [September 8, 2025, 4:34pm UTC](https://forum.cadet-web.de/t/fitting-reaction-parameters-to-a-set-of-experimental-data/1296 "2025-09-08T16:34:03Z")
Hi everyone, I am currently trying to simulate a reactive chromatographic process. In this a reaction should occur in the solid phase. For this I would like to fit parameters of the reaction system (k\_fwd, k\_bwd) to a s… --- ## [Pore and Film Diffusion and Multicomponent Colloidal Model](https://forum.cadet-web.de/t/pore-and-film-diffusion-and-multicomponent-colloidal-model/1309)
**Author:** [@sasha\_onfleek1](https://forum.cadet-web.de/u/sasha_onfleek1)\ **Replies:** 0\ **Last updated:** [September 8, 2025, 2:34pm UTC](https://forum.cadet-web.de/t/pore-and-film-diffusion-and-multicomponent-colloidal-model/1309 "2025-09-08T14:34:49Z")
Hello everyone! I am fitting my ALC gradient elution data using the Multi Component Colloidal model and the GRM model with success. However I have a question regarding the Pore and Film Diffusion. I’m simulating the elu… --- ## [Multi State Steric Mass Action (MSSMA) Isotherm Ka and Kd Units](https://forum.cadet-web.de/t/multi-state-steric-mass-action-mssma-isotherm-ka-and-kd-units/1306)
**Author:** [@Will\_m32](https://forum.cadet-web.de/u/Will_m32)\ **Replies:** 2\ **Last updated:** [September 7, 2025, 12:08am UTC](https://forum.cadet-web.de/t/multi-state-steric-mass-action-mssma-isotherm-ka-and-kd-units/1306 "2025-09-07T00:08:55Z")
Hi There, I have a question around the units used for simulation of the MSSMA in CADET-Process. When simulating the MSSMA in cadet-process (using reference concentrations) are the rate constant values that I input into… --- ## [CADET not found (new thread September 2025)](https://forum.cadet-web.de/t/cadet-not-found-new-thread-september-2025/1304)
**Author:** [@AN\_IA](https://forum.cadet-web.de/u/AN_IA)\ **Replies:** 3\ **Last updated:** [September 5, 2025, 6:48pm UTC](https://forum.cadet-web.de/t/cadet-not-found-new-thread-september-2025/1304 "2025-09-05T18:48:22Z")
Expected behavior. Hi everyone, as requested, I’m starting a new thread with my problem, which I first described in this thread: Expected behavior: (1) CADET should be able to reliably autodetect its own installati… --- ## [Cadet not found](https://forum.cadet-web.de/t/cadet-not-found/1063)
**Author:** [@akshayts](https://forum.cadet-web.de/u/akshayts)\ **Replies:** 6\ **Last updated:** [September 3, 2025, 12:24pm UTC](https://forum.cadet-web.de/t/cadet-not-found/1063 "2025-09-03T12:24:01Z")
Hi, I am fairly new to this platform. I have installed CADET-Process and Cadet on VisualStudio code. I am trying to run the CadetProcess simulator and when I run this command - from CADETProcess.simulator import Cadet … --- ## [How to modify model equations and inspect used equations per model in CADET?](https://forum.cadet-web.de/t/how-to-modify-model-equations-and-inspect-used-equations-per-model-in-cadet/1300)
**Author:** [@virginioaioan](https://forum.cadet-web.de/u/virginioaioan)\ **Replies:** 1\ **Last updated:** [August 30, 2025, 10:00am UTC](https://forum.cadet-web.de/t/how-to-modify-model-equations-and-inspect-used-equations-per-model-in-cadet/1300 "2025-08-30T10:00:41Z")
Hello I’d like to understand in detail which specific equations are used for different models in CADET (e.g., binding or transport models) and modify them by myself. Do I need to build CADET-Core from source to access … [Previous page](https://forum.cadet-web.de/latest.md) [Next page](https://forum.cadet-web.de/latest.md?page=2)