Workflow for modelling unknown separation in reversed-phase chromatography

Hello and welcome to the forum!

Great to hear you’re getting started with CADET-Process! The general steps you’ve outlined are correct, though still high-level.

Regarding the choice of isotherm, this depends on your specific experimental setup and the adsorption behavior of your components. CADET itself doesn’t determine the isotherm for you—you’ll need to select an appropriate model based on your system. More details on available isotherms can be found in the CADET-Core documentation.

Converting absorbance to concentration usually requires a calibration experiment. Someone in your lab can likely help you determine the extinction coefficient for your compound.

Until these process-specific decisions are made and the necessary data is gathered on your end, I am afraid there is not much we can do to support implementing your setup into CADET-Process.

If you’re interested in learning more about applying CADET-Process to specific setups and getting hands-on guidance, you could consider joining the next CADET Workshop! It’s a great opportunity to deepen your understanding and ask questions directly.
I would also like to mention the CADET Office hours, where you can always swing by if you find yourself stuck while implementing CADET.

Hope that already helps!

Best regards,
Hannah

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