# \#application **URL:** https://forum.cadet-web.de/tag/application/1.md [Latest](https://forum.cadet-web.de/latest.md) · [Categories](https://forum.cadet-web.de/categories.md) · [Tags](https://forum.cadet-web.de/tags.md) --- ## [Modeling reverse phase chromatography by CADET](https://forum.cadet-web.de/t/modeling-reverse-phase-chromatography-by-cadet/1329)
**Author:** [@chenkangnan](https://forum.cadet-web.de/u/chenkangnan)\ **Replies:** 15\ **Last updated:** [October 9, 2026, 7:15am UTC](https://forum.cadet-web.de/t/modeling-reverse-phase-chromatography-by-cadet/1329 "2026-10-09T07:15:23Z")
Hello everyone, Has anyone attempted to model the separation of peptides by reverse phase chromatography using CADET? What model is commonly used in CADET?I have chosen the Mobile Phase Modulator Langmuir (MPM) model, b… --- ## [Examples to use gACT isotherm for (multimodal) cation exchange chromatography with split peaks](https://forum.cadet-web.de/t/examples-to-use-gact-isotherm-for-multimodal-cation-exchange-chromatography-with-split-peaks/1428)
**Author:** [@Flynn](https://forum.cadet-web.de/u/Flynn)\ **Replies:** 1\ **Last updated:** [May 18, 2026, 8:30pm UTC](https://forum.cadet-web.de/t/examples-to-use-gact-isotherm-for-multimodal-cation-exchange-chromatography-with-split-peaks/1428 "2026-05-18T20:30:20Z")
Background As I discussed in the Recovery conference, the design philosophy of ACT isotherm goes far beyond affinity chromatography. In theory, it can be applied to any modes of chromatography as detailed in the paper Ap… --- ## [Fitting reaction parameters to a set of experimental data](https://forum.cadet-web.de/t/fitting-reaction-parameters-to-a-set-of-experimental-data/1296)
**Author:** [@Adrian](https://forum.cadet-web.de/u/Adrian)\ **Replies:** 9\ **Last updated:** [September 8, 2025, 4:34pm UTC](https://forum.cadet-web.de/t/fitting-reaction-parameters-to-a-set-of-experimental-data/1296 "2025-09-08T16:34:03Z")
Hi everyone, I am currently trying to simulate a reactive chromatographic process. In this a reaction should occur in the solid phase. For this I would like to fit parameters of the reaction system (k\_fwd, k\_bwd) to a s… --- ## [Cadet not found](https://forum.cadet-web.de/t/cadet-not-found/1063)
**Author:** [@akshayts](https://forum.cadet-web.de/u/akshayts)\ **Replies:** 6\ **Last updated:** [September 3, 2025, 12:24pm UTC](https://forum.cadet-web.de/t/cadet-not-found/1063 "2025-09-03T12:24:01Z")
Hi, I am fairly new to this platform. I have installed CADET-Process and Cadet on VisualStudio code. I am trying to run the CadetProcess simulator and when I run this command - from CADETProcess.simulator import Cadet … --- ## [Question about protA model using CADET python](https://forum.cadet-web.de/t/question-about-prota-model-using-cadet-python/1197)
**Author:** [@kautharmaalim](https://forum.cadet-web.de/u/kautharmaalim)\ **Replies:** 6\ **Last updated:** [May 1, 2025, 6:09am UTC](https://forum.cadet-web.de/t/question-about-prota-model-using-cadet-python/1197 "2025-05-01T06:09:14Z")
def get\_cadet\_template(n\_units=3, split\_components\_data=True): cadet\_template = Cadet() cadet\_template.root.input.model.nunits = n\_units # Store solution cadet\_template.root.input\['return'\].split\_com… --- ## [Simulation failed using HIC isotherm](https://forum.cadet-web.de/t/simulation-failed-using-hic-isotherm/1199)
**Author:** [@Yang](https://forum.cadet-web.de/u/Yang)\ **Replies:** 2\ **Last updated:** [April 13, 2025, 1:43pm UTC](https://forum.cadet-web.de/t/simulation-failed-using-hic-isotherm/1199 "2025-04-13T13:43:24Z")
Expected behavior. Hi everyone, I am trying to use HICConstantWaterActivity isotherm. I found an unexpect phenomenon, when I added a ‘CSTR’ the simulation never stops, but when I annotated “CSTR” code, the simulation su… --- ## [Some questions about reverse phase chromatography modeling](https://forum.cadet-web.de/t/some-questions-about-reverse-phase-chromatography-modeling/1187)
**Author:** [@chenkangnan](https://forum.cadet-web.de/u/chenkangnan)\ **Replies:** 9\ **Last updated:** [April 3, 2025, 10:08am UTC](https://forum.cadet-web.de/t/some-questions-about-reverse-phase-chromatography-modeling/1187 "2025-04-03T10:08:21Z")
Hello everyone, I just learned the CADET-Process recently and feel its powerful ability. I am currently using reverse phase chromatography to separate an industrial peptide. Mobile phase A is a salt , and mobile phase … --- ## [Cadet Match type and zero division errors in parameter estimation](https://forum.cadet-web.de/t/cadet-match-type-and-zero-division-errors-in-parameter-estimation/1111)
**Author:** [@nbarry03](https://forum.cadet-web.de/u/nbarry03)\ **Replies:** 4\ **Last updated:** [November 28, 2024, 5:13pm UTC](https://forum.cadet-web.de/t/cadet-match-type-and-zero-division-errors-in-parameter-estimation/1111 "2024-11-28T17:13:29Z")
Hi, I am attempting to estimate some parameters using Match, however I have been running into a a couple of errors related to one of my inlet units when I attempt to run the parameter estimation. The model is built with… --- ## [Adapting GRM to membrane chromatography](https://forum.cadet-web.de/t/adapting-grm-to-membrane-chromatography/1094)
**Author:** [@rosario.cervellere](https://forum.cadet-web.de/u/rosario.cervellere)\ **Replies:** 4\ **Last updated:** [November 13, 2024, 9:04pm UTC](https://forum.cadet-web.de/t/adapting-grm-to-membrane-chromatography/1094 "2024-11-13T21:04:56Z")
I want to utilize cadet for simulating membrane chromatography. In doing so I need to get under the hood and make a few changes to the GRM and underlying assumptions. Are there readily available resources that outline th… --- ## [Trouble with understanding "storage" concentrations in channels without flow (MCT model)](https://forum.cadet-web.de/t/trouble-with-understanding-storage-concentrations-in-channels-without-flow-mct-model/985)
**Author:** [@h.lanzrath](https://forum.cadet-web.de/u/h.lanzrath)\ **Replies:** 2\ **Last updated:** [July 24, 2024, 10:54am UTC](https://forum.cadet-web.de/t/trouble-with-understanding-storage-concentrations-in-channels-without-flow-mct-model/985 "2024-07-24T10:54:14Z")
Hello, I have encountered some issues when fitting my data and it led me down a rabbit hole of confusion and what I think are mistakes in some general assumptions I had. I will start in the beginning: I am using the M… --- ## [CADET Matlab Installation](https://forum.cadet-web.de/t/cadet-matlab-installation/339)
**Author:** [@franziska.hagemann](https://forum.cadet-web.de/u/franziska.hagemann)\ **Replies:** 6\ **Last updated:** [June 10, 2024, 12:36pm UTC](https://forum.cadet-web.de/t/cadet-matlab-installation/339 "2024-06-10T12:36:44Z")
Dear all, Unfortunately I am having trouble to excecute the installCADET.m file. In the command window I get the following information: “Unfortunately, no working CADET interface could be found.” I remember that one ha… --- ## [LeBarre et al. (2024): Mixed-mode size-exclusion silica resin for polishing human antibodies in flow-through mode](https://forum.cadet-web.de/t/lebarre-et-al-2024-mixed-mode-size-exclusion-silica-resin-for-polishing-human-antibodies-in-flow-through-mode/889)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [February 29, 2024, 7:58pm UTC](https://forum.cadet-web.de/t/lebarre-et-al-2024-mixed-mode-size-exclusion-silica-resin-for-polishing-human-antibodies-in-flow-through-mode/889 "2024-02-29T19:58:04Z")
LeBarre, J.; Chu, W.; Altern, S.; Kocot, A.; Bhandari, D.; Barbieri, E.; Sly, J.; Crapanzano, M.; Cramer, S.; Phillips, M.; Roush, D.; Carbonell, R.; Boi, C.; Menegatti, S.: Mixed-mode size-exclusion silica resin for pol… --- ## [Rischawy et al. (2023): Connected mechanistic process modeling to predict a commercial biopharmaceutical downstream process](https://forum.cadet-web.de/t/rischawy-et-al-2023-connected-mechanistic-process-modeling-to-predict-a-commercial-biopharmaceutical-downstream-process/696)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [May 25, 2023, 11:08am UTC](https://forum.cadet-web.de/t/rischawy-et-al-2023-connected-mechanistic-process-modeling-to-predict-a-commercial-biopharmaceutical-downstream-process/696 "2023-05-25T11:08:19Z")
Rischawy, F.; Briskot, T.; Hopf, N.; Saleh, D.; Wang, G.; Kluters, S.; Studts, J.; Hubbuch, J.: Connected mechanistic process modeling to predict a commercial biopharmaceutical downstream process, Computers & Chemical En… --- ## [Heymann et al. (2023): Advanced error modeling and Bayesian uncertainty quantification in mechanistic liquid chromatography modeling](https://forum.cadet-web.de/t/heymann-et-al-2023-advanced-error-modeling-and-bayesian-uncertainty-quantification-in-mechanistic-liquid-chromatography-modeling/770)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [September 13, 2023, 5:25pm UTC](https://forum.cadet-web.de/t/heymann-et-al-2023-advanced-error-modeling-and-bayesian-uncertainty-quantification-in-mechanistic-liquid-chromatography-modeling/770 "2023-09-13T17:25:55Z")
Heymann, W.; Glaser, J.; Schlegel, F.; Johnson, W.; Rolandi, P.; von Lieres, E.: Advanced error modeling and Bayesian uncertainty quantification in mechanistic liquid chromatography modeling, Journal of Chromatography A… --- ## [Yang et al. (2024): Parameter-by-parameter estimation method for adsorption isotherm in hydrophobic interaction chromatography](https://forum.cadet-web.de/t/yang-et-al-2024-parameter-by-parameter-estimation-method-for-adsorption-isotherm-in-hydrophobic-interaction-chromatography/861)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [January 27, 2024, 11:46am UTC](https://forum.cadet-web.de/t/yang-et-al-2024-parameter-by-parameter-estimation-method-for-adsorption-isotherm-in-hydrophobic-interaction-chromatography/861 "2024-01-27T11:46:51Z")
Yang, Y.-X.; Chen, Y.-C.; Yao, S.-J.; Lin, D.-Q.: Parameter-by-parameter estimation method for adsorption isotherm in hydrophobic interaction chromatography, Journal of Chromatography A 1716,8 (2024), 464638. CADET used… --- ## [Ughade et al. (2024): Mechanistic modeling of size exclusion chromatography-assisted in vitro refolding of the recombinant biosimilar teriparatide (PTH-34)](https://forum.cadet-web.de/t/ughade-et-al-2024-mechanistic-modeling-of-size-exclusion-chromatography-assisted-in-vitro-refolding-of-the-recombinant-biosimilar-teriparatide-pth-34/866)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [February 5, 2024, 1:24pm UTC](https://forum.cadet-web.de/t/ughade-et-al-2024-mechanistic-modeling-of-size-exclusion-chromatography-assisted-in-vitro-refolding-of-the-recombinant-biosimilar-teriparatide-pth-34/866 "2024-02-05T13:24:15Z")
Ughade, S.; Rana, S.; Nadeem, M.; Kumthekar, R.; Mahajani, S.; Bhambure, R.: Mechanistic modeling of size exclusion chromatography-assisted in vitro refolding of the recombinant biosimilar teriparatide (PTH-34), ACS Omeg… --- ## [Frandsen et al. (2024):Shortcut design method for multicomponent gradient simulated moving beds](https://forum.cadet-web.de/t/frandsen-et-al-2024-shortcut-design-method-for-multicomponent-gradient-simulated-moving-beds/806)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [November 21, 2023, 8:32pm UTC](https://forum.cadet-web.de/t/frandsen-et-al-2024-shortcut-design-method-for-multicomponent-gradient-simulated-moving-beds/806 "2023-11-21T20:32:24Z")
Frandsen, J.; Huusom, J.; Gernaey, K.; Abildskov, J.: Shortcut design method for multicomponent gradient simulated moving beds, AIChE Journal 70,2 (2024): e18304. CADET used to verify a shortcut design method for SMB un… --- ## [Diehm et al. (2024): Micro simulated moving bed chromatography-mass spectrometry as a continuous on-line process analytical tool](https://forum.cadet-web.de/t/diehm-et-al-2024-micro-simulated-moving-bed-chromatography-mass-spectrometry-as-a-continuous-on-line-process-analytical-tool/807)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [November 21, 2023, 8:35pm UTC](https://forum.cadet-web.de/t/diehm-et-al-2024-micro-simulated-moving-bed-chromatography-mass-spectrometry-as-a-continuous-on-line-process-analytical-tool/807 "2023-11-21T20:35:58Z")
Diehm, J.; Witting, L.; Kirschhöfer, F.; Brenner-Weiß, G.; Franzreb, M.: Micro simulated moving bed chromatography-mass spectrometry as a continuous on-line process analytical tool, Analytical and Bioanalytical Chemistry… --- ## [Tang et al. (2023): Physics-informed neural networks to solve lumped kinetic model for chromatography process](https://forum.cadet-web.de/t/tang-et-al-2023-physics-informed-neural-networks-to-solve-lumped-kinetic-model-for-chromatography-process/769)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [September 12, 2023, 4:10pm UTC](https://forum.cadet-web.de/t/tang-et-al-2023-physics-informed-neural-networks-to-solve-lumped-kinetic-model-for-chromatography-process/769 "2023-09-12T16:10:08Z")
Tang, S.-Y.; Yuan, Y.-H.; Chen, Y.-C.; Yao, S.-J.; Wang, Y.; Lin, D.-Q.: Physics-informed neural networks to solve lumped kinetic model for chromatography process, Journal of Chromatography A 1708 (2023): 464346. CADET … --- ## [Ding et al. (2024): Machine learning-based optimization of a multi-step ion exchange chromatography for ternary protein separation](https://forum.cadet-web.de/t/ding-et-al-2024-machine-learning-based-optimization-of-a-multi-step-ion-exchange-chromatography-for-ternary-protein-separation/887)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [February 26, 2024, 4:18pm UTC](https://forum.cadet-web.de/t/ding-et-al-2024-machine-learning-based-optimization-of-a-multi-step-ion-exchange-chromatography-for-ternary-protein-separation/887 "2024-02-26T16:18:22Z")
Ding, C.; Ierapetritou, M.: Machine learning-based optimization of a multi-step ion exchange chromatography for ternary protein separation, Computers & Chemical Engineering, in press. --- ## [Rao et al. (2023): High definition simulation of packed bed chromatography](https://forum.cadet-web.de/t/rao-et-al-2023-high-definition-simulation-of-packed-bed-chromatography/747)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [July 27, 2023, 4:24pm UTC](https://forum.cadet-web.de/t/rao-et-al-2023-high-definition-simulation-of-packed-bed-chromatography/747 "2023-07-27T16:24:31Z")
Rao, J.; Püttmann, A.; Khirevich, S.; Tallarek, U.; Geuzaine, C.; Behr, M.; von Lieres, E.: High definition simulation of packed bed chromatography, Computers & Chemical Engineering 178 (2023): 108355. The title is prog… --- ## [He et al. (2018): Efficient numerical simulation of simulated moving bed chromatography with a single-column solver](https://forum.cadet-web.de/t/he-et-al-2018-efficient-numerical-simulation-of-simulated-moving-bed-chromatography-with-a-single-column-solver/37)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [June 4, 2020, 8:55am UTC](https://forum.cadet-web.de/t/he-et-al-2018-efficient-numerical-simulation-of-simulated-moving-bed-chromatography-with-a-single-column-solver/37 "2020-06-04T08:55:11Z")
He, Q.-L.; Leweke, S.; von Lieres, E.: Efficient numerical simulation of simulated moving bed chromatography with a single-column solver, Computers & Chemical Engineering 111 (2018): 183–198. Cyclic multi-column network… --- ## [He et al. (2020): Model-based process design of a ternary protein separation using multi-step gradient ion-exchange SMB chromatography](https://forum.cadet-web.de/t/he-et-al-2020-model-based-process-design-of-a-ternary-protein-separation-using-multi-step-gradient-ion-exchange-smb-chromatography/36)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [June 4, 2020, 8:53am UTC](https://forum.cadet-web.de/t/he-et-al-2020-model-based-process-design-of-a-ternary-protein-separation-using-multi-step-gradient-ion-exchange-smb-chromatography/36 "2020-06-04T08:53:49Z")
He, Q.-L.; von Lieres, E.; Zhao, L.; Sun, Z.: Model-based process design of a ternary protein separation using multi-step gradient ion-exchange SMB chromatography, Computers & Chemical Engineering 138 (2020): 106851. Se… --- ## [Altern et al. (2024): Mechanistic model-based characterization of size-exclusion-mixed-mode resins for removal of monoclonal antibody fragments](https://forum.cadet-web.de/t/altern-et-al-2024-mechanistic-model-based-characterization-of-size-exclusion-mixed-mode-resins-for-removal-of-monoclonal-antibody-fragments/873)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 1\ **Last updated:** [February 19, 2024, 1:12am UTC](https://forum.cadet-web.de/t/altern-et-al-2024-mechanistic-model-based-characterization-of-size-exclusion-mixed-mode-resins-for-removal-of-monoclonal-antibody-fragments/873 "2024-02-19T01:12:19Z")
Altern, S.; Kocot, A.; LeBarre, J.; Boi, C.; Phillips, M.; Roush, D.; Menegatti, S.; Cramer, S.: Mechanistic model-based characterization of size-exclusion-mixed-mode resins for removal of monoclonal antibody fragments, … --- ## [Bhoyar et al. (2024): Predictive mechanistic modeling of loading and elution in protein A chromatography](https://forum.cadet-web.de/t/bhoyar-et-al-2024-predictive-mechanistic-modeling-of-loading-and-elution-in-protein-a-chromatography/828)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 2\ **Last updated:** [January 19, 2024, 3:40pm UTC](https://forum.cadet-web.de/t/bhoyar-et-al-2024-predictive-mechanistic-modeling-of-loading-and-elution-in-protein-a-chromatography/828 "2024-01-19T15:40:18Z")
Bhoyar, S.; Kumar, V.; Foster, M.; Xu, X.; Traylor, S.; Guo, J.; Lenhoff, A.: Predictive mechanistic modeling of loading and elution in protein A chromatography, Journal of Chromatography A 1713 (2024): 464558. CADET us… --- ## [Example for parameter estimation using multiple experiments](https://forum.cadet-web.de/t/example-for-parameter-estimation-using-multiple-experiments/822)
**Author:** [@ronald.jaepel](https://forum.cadet-web.de/u/ronald.jaepel)\ **Replies:** 0\ **Last updated:** [December 7, 2023, 3:55pm UTC](https://forum.cadet-web.de/t/example-for-parameter-estimation-using-multiple-experiments/822 "2023-12-07T15:55:37Z")
Hello, I’ve added an example on how to determine SMA parameters based on multiple gradient elutions here. It is based on a recent fix to the CADET-Process code and requires this branch to function. It will hopefully be … --- ## [Example for parameter estimation using the Yamamoto method](https://forum.cadet-web.de/t/example-for-parameter-estimation-using-the-yamamoto-method/821)
**Author:** [@ronald.jaepel](https://forum.cadet-web.de/u/ronald.jaepel)\ **Replies:** 0\ **Last updated:** [December 7, 2023, 3:52pm UTC](https://forum.cadet-web.de/t/example-for-parameter-estimation-using-the-yamamoto-method/821 "2023-12-07T15:52:59Z")
Hello everyone, I’ve expanded the CADET-Process examples library to include examples on the estimation of SMA binding parameters using the Yamamoto method. It is currently located here and will be merged into the dev br… --- ## [Kumar et al. (2015): Mechanistic modeling of ion-exchange process chromatography of charge variants of monoclonal antibody products](https://forum.cadet-web.de/t/kumar-et-al-2015-mechanistic-modeling-of-ion-exchange-process-chromatography-of-charge-variants-of-monoclonal-antibody-products/45)
**Author:** [@s.leweke](https://forum.cadet-web.de/u/s.leweke)\ **Replies:** 0\ **Last updated:** [June 9, 2020, 9:14pm UTC](https://forum.cadet-web.de/t/kumar-et-al-2015-mechanistic-modeling-of-ion-exchange-process-chromatography-of-charge-variants-of-monoclonal-antibody-products/45 "2020-06-09T21:14:28Z")
Kumar, V.; Leweke, S.; von Lieres, E.; Rathore, A.: Mechanistic modeling of ion-exchange process chromatography of charge variants of monoclonal antibody products, Journal of Chromatography A 1426 (2015): 140–153. Param… --- ## [Hagemann et al. (2020): Modeling hindered diffusion of antibodies in agarose beads considering pore size reduction due to adsorption](https://forum.cadet-web.de/t/hagemann-et-al-2020-modeling-hindered-diffusion-of-antibodies-in-agarose-beads-considering-pore-size-reduction-due-to-adsorption/68)
**Author:** [@lieres](https://forum.cadet-web.de/u/lieres)\ **Replies:** 0\ **Last updated:** [June 18, 2020, 4:21pm UTC](https://forum.cadet-web.de/t/hagemann-et-al-2020-modeling-hindered-diffusion-of-antibodies-in-agarose-beads-considering-pore-size-reduction-due-to-adsorption/68 "2020-06-18T16:21:45Z")
Hagemann, F.; Adametz, P.; Wessling, M.; Thom, V.: Modeling hindered diffusion of antibodies in agarose beads considering pore size reduction due to adsorption, Journal of Chromatography A 1626 (2020): 461319. Model-bas… --- ## [Questions about Using CADET and GoSilico in Biopharmaceutical Purification Proces](https://forum.cadet-web.de/t/questions-about-using-cadet-and-gosilico-in-biopharmaceutical-purification-proces/720)
**Author:** [@jaymax](https://forum.cadet-web.de/u/jaymax)\ **Replies:** 3\ **Last updated:** [July 10, 2023, 1:49pm UTC](https://forum.cadet-web.de/t/questions-about-using-cadet-and-gosilico-in-biopharmaceutical-purification-proces/720 "2023-07-10T13:49:59Z")
Hi I am managing the purification process of biopharmaceuticals in a factory, and I need a mechanistic modeling tool, so I am looking for several sources. I recently discovered CADET and after reading the papers, it see… [Next page](https://forum.cadet-web.de/tag/application/1.md?match_all_tags=true&page=1&tags%5B%5D=application)