Hi!
I am totally new in CADET. However I was encouraged to learn CADET since we think that it can help us to see deeper in our chromatography process, hoping to perform a better separation for our target protein.
We have several questions as we are working with this platform to look at the Kads and Kdes of our sample using HIC.
- We’re wondering whether limitations are exist regarding protein concentration data which we input. The value of our concentration data (both target and impurities) is quite low (~0.02-2 mg/mL), and we’re afraid that too low concentration may not be proper to be used.
- Would it be possible for us to look at kads / kdes when our target is contained in a sample mixture, where the ‘impurities’ have similar properties to our target? (example: a pool of sample after undergoing digestion process, hence it contains initial substrate, intermediate products, protein target (around 60-70%), and nonspecific digested product). We’re asking since our team cannot acquire kads/kdes from the data we put in.
- Is there non ideal conditions (specifically on the sample properties) where it’s not probable to look at the kads/kdes?
Thank you in advance. We’re open to suggestions & corrections.