pH dependent isotherm parameter for Langmuir LDF

[Flynn]

You could also try the ACT isotherm in this post:

An example to use ACT isotherm for protein A affinity chromatography

Introduction The ACT isotherm is a modified Langmuir isotherm tailored for mechanistic modeling in protein A chromatography. It is known that both binding capacity q_{max} and equilibrium constant (affinity) K_{eq} changes as a function of pH. ACT isotherm uses modification factors derived based on proA and mAb interaction stoichiometries. Details can be found in the paper. [ACT isotherm concept.] [ACT isotherm behaviors.] Example code from cadet import Cadet import numpy as np import matpl…

It will be available in the next release shortly.

One problem with mobile phase modulator Langmuir is that it cannot model q_{max} changes as a function of pH. ACT isotherm is capable of doing it.